In the UK, there are parties represented in regional and the national parliament calling for either the secession of, or at least greater autonomy for their nations from the UK (e.g. SNP, Plaid Cymru, etc.) In Spain I believe something like 40-60% of parliamentary seats in Catalonia and the Basque country are regularly won by parties similarly calling for secession / greater autonomy for those lands. I know of regional autonomy movements which have achieved political representation in Italy too, and I believe at least some of the mainstream political parties in Belgium call for the independence of Flanders/Wallonia.
Are there any comparable regionalist parties in Germany - i.e. calling for greater autonomy for any single states, or for a complete secession of a state from the federal government, who have been able to achieve seats in the Bundestag, regional or EU parliaments?