Before 1955
I don't know enough about UK politics to know whether Conservative and Labour were both major parties, but in 1918 the Conservatives received 1.1% of the vote in Pontypridd, according to the results here.
Since 1955
(Original Answer) Excepting constituencies where the Speaker was standing for reelection, I do not believe this has happened since 1955 based on the data here, but perhaps an election at 2.2% is close enough?
Those data provide the total population, but here are the three lowest performances for either party:
Year |
Constituency |
Electorate / Turnout |
Conservative (%) |
Labour (%) |
2010 |
Westmorland and Lonsdale |
67,881 / 51,487 |
18,632 (36.2) |
1,158 (2.2) |
2005 |
Blaenau Gwent |
53,301 / 35,251 |
816 (2.4) |
11,384 (32.3) |
1983 |
Isle of Wight |
94,226 / 75,347 |
34,901 (51.0) |
1,828 (2.4) |