I don't think it's possible since there are no more Greeks in Turkey, both because the Greek genocide, perpetrated by the Ottoman Empire, and for the subsequent Population exchange between Greece and Turkey.
There are some Greeks that still support the Megali idea, that is to say the recovery of the old greeks lands, usually in the far right, but they don't live in East Thrace.
As Anixx said in a comment, in theory it's possible that there coulde be a separatist movement not driven by Greeks, but a common (East-)Thracian identity. In practice, though, given the historical hostility between Greece and the Ottoman Empire/Turkey it would be very hard to create a separatist movement in that region that isn't automatically seen as a pro-Greece movement and thus a non-starter.
Before the recent decades of economic growth there could have been some space for a urban elite against rural people type of movement in East Thrace and Instabul. Some of this divide had a role in the rise of Erdogan, who was greatly supported by the more religious rural people. But today there is a more even demographic distribution.
For the creation of a separatist movement there must be a strong unique identity and I don't see that in East Thrace.